KcOiUvzgOn clWYkuKNRegIGfsYTI IIVgCHDgEAzdKfwIXK  
KcOiUvzgOn clWYkuKNRegIGfsYTI IIVgCHDgEAzdKfwIXK
KcOiUvzgOn clWYkuKNRegIGfsYTI IIVgCHDgEAzdKfwIXK
Lepti Trim inc
KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK THE LEPTIN FACTOR The Case of the Amazing Shrinking Fat Cell After using Lepti-Trim6 with leptin from Immune Tree, I can tell you that I have not only lost weight. I have also experienced a dramatic conversion of body mass from fat to muscle. Perhaps most noteworthy (and certainly delightful to this chocolate lover), is that my taste for sweets has been significantly blunted. Researchers say that is a beneficial property of this hormone-like protein. They’re right. U sing ger your the hormone-like messen- protein leptin to shrink fat cells is an intriguing, potentially key new concept in weight loss. Diminishing the body’s cravings that lead to overeating is another criti- cal, new concept. And with more and more of us din- ing out on, or taking out, industrial- sized meal portions, it is clear that shrinking our fat cells and reducing cravings that lead to overeating are potentially two very good things. So what if we really could safely shrink our fat cells (without losing muscle)? What if we simply could safely lose the cravings that cause us to overeat? The science of slimming is now on the verge of such breakthroughs. We cannot assure everyone reading this report that the information we pre- sent will result in weight loss or con- version of fat to lean muscle, because the information is preliminary (and also individual biochemistry affects all outcomes). KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK But, many of us welcome all the help we can get when it comes to appetite control and weight loss. Our mission is to keep you informed of cutting edge breakthroughs in health. Anecdotal reports are coming in now from individuals who are losing weight and/or becoming more trim Leptin Background FYI Leptin, a hormone-like messenger protein (known with the use of a leptin-based colostrum formula from Immune Tree. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK It is called Lepti-Trim6. technically as a cytokine), is produced in relatively large amounts by fat tissue and in smaller amounts by other peripheral organs, and then secreted into the bloodstream, where it travels to the brain and other tissues, causing fat loss and decreased appetite. So we want to present this information. Once leptin has been secreted by your fat cells, it travels to the hypothalamus—the It may prove helpful to many of you. part of your brain that has an influence on eating behavior. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK In the medial hypothala- mus, leptin activates “anorectic” nerve cells, which serve to suppress your appetite. At Scientists Say Leptin Works As a longtime consultant to the dairy industry, Dr. Al Fox has seen firsthand the beneficial impact of leptin on body mass composition. “I have been the same time, leptin prevents “orexigenic” cells from stimulating your appetite. Leptin (from the Greek leptos, meaning thin) first came to national attention in 1994 when the obesity gene and its product leptin were discovered. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK It was shown then that obese mice dropped 40 percent of their body weight after only one month of treatment with leptin. Leptin also improved symptoms of diabetes. Recently, the taste organ was found to be one of the peripheral targets for leptin. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK The hormone specifically inhibits sweet taste responses in lean mice. Thus, leptin involved in studies with lean body appears to act as a modulator of sweet taste. mass and calves, and we have demon- strated that supplemental colostrum aids lean body mass. It seems likely Leptin also suppresses a gene that produces an enzyme known as acetyl-CoA carboxylase, or ACC, which is essential for fat production. When researchers injected laboratory mice with leptin, previously obese mice became as thin as rodent track stars. Some researchers believe that leptin works, in part, by inhibiting the synthesis of fat in fat cells and increasing the burning of fat in muscle cells—that 36 The Doctors’ Prescription for Healthy Living / Volume 6, Number 6 it works at an enzymatic, cellular level. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK that it is the combination of leptin and have been using genechip technol- insulinlike growth factor-I (IGF-I) in ogy—a powerful tool for analyzing the colostrum that helps to create lean expression patterns of thousands of body mass,” says Fox, one of the genes at a time—and have identified a world’s leading experts on colostrum, number of genes that are specifically its formulation, utilization and applica- regulated by the hormone leptin. tions. Dr. Fox is scientific consultant to Identifying genes regulated by lep- Immune Tree. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK tin will improve knowledge of how “Leptin directs how you actually leptin causes its effects on weight and use fatty acids,” he continues. “Large appetite, and may also offer new tar- fat cells indicate a leptin deficiency or gets for drugs designed to stimulate an inability to properly use leptin due weight loss. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Since the discovery of to faulty cell receptors or other feed- leptin in 1994, many have hoped that back mechanisms. It’s a vicious cycle. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK the hormone would be a promising Once you get a lot of large fat cells you weight-loss treatment for humans. have inefficient uses of leptin and you Studies of the hormone’s weight- end up with more fat cells and even reducing effects in humans are more leptin because your body is no underway, but researchers still have longer responding. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK But as people lose a way to go before they fully compre- weight, their leptin levels decrease, hend how the hormone affects the making it difficult to reach ideal body brain and other tissues. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK weight. What we do know is that sup- In experiments described in the plemental leptin seems to work.” April 15, 2000, issue of the journal Most telling is a 1999 study from the Genes & Development, Dr. Jeffrey M. Journal of the American Medical Friedman, an HHMI investigator at Association that found that leptin not The Rockefeller University, and only resulted in significant weigh loss, Rockefeller colleagues Alexander “more than 95% of weight loss was fat Soukas, Paul Cohen and Nicholas D. loss,” note researchers. Socci report that they are beginning to Meanwhile, researchers at the probe the genetic program orches- Howard Hughes Medical Institute trated by leptin to induce weight loss. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK THE LEPTIN FACTOR cont inued Most telling is a 1999 study from the Journal of the American Medical Association that found that leptin not only resulted in significant weight loss, “more than 95% of weight loss was fat loss,” note researchers. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Prescription for Healthy Living Lepti-Trim6, a formula with the isolated leptin molecule in a colostrum base, also supplies additional lep- tin and insulinlike growth factor-I, which may also aid the body in weight loss and conversion of fat mass to lean muscle. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK It’s too early for us to give you a definitive word on how well Lepti-Trim6 works and in what percentage of users. But the formula looks really promising. Anecdotal reports have been coming in which also show promise. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Our mission is to keep you on the cutting edge and get you the information you need first. We know that the formula is safe and we have reason to believe it works. Availability—Immune Tree Leptri-Trim6 is available through natural health distributers nationwide. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK If you have any trouble finding a source for it, call Immune Tree toll-free at (877) 295-1269 or go to one of their online vendors www.dockofhealth.com. Volume 6, Number 6 / The Doctors’ Prescription for Healthy Living 37 “We knew that an animal given lep- tin eats less and loses fat,” says Dr. Friedman. “And while restricting food intake also causes weight loss, we had reason to believe that the two weight- loss responses are very different.” KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK For example, says Dr. Friedman, leptin triggers weight-loss of fat stores alone, while food-restriction robs the body of both fat and muscle. Also, he says, a diet-restricted human or animal com- pensates for decreased caloric intake by lowering energy expenditure, while leptin treatment shows no such energy-robbing effect. Until now, how- ever, no one had explored the molecu- lar basis of such differences in detail. Studying normal mice and a mutant strain that cannot produce leptin, the researchers looked for differences in gene expression patterns related to either leptin administration or caloric restriction. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK After administering leptin to or restricting food intake in the two groups of mice, the researchers ana- lyzed gene expression in the mice by extracting messenger RNA from their fat cells. Messenger RNA levels reflect the expression levels of different genes. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK They applied these collections of messenger RNA to a series of “oligonucleotide microarrays,” popu- larly known as genechips. Each kind of messenger RNA “found” and adhered to its corresponding gene on the genechip. Indicator molecules revealed the level of RNA present, showing the expression levels of hun- dreds of fat-tissue-related genes. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Analyzing data from many such experiments with the mice, the scien- tists were able to group the expressed genes into clusters that appeared to behave similarly— increasing or decreasing in expression in tandem— as the mice were subjected to differ- ent regimes of leptin treatment or food restriction. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK “We were able to find at least half a dozen distinct clusters of genes that were specifically regulated by leptin and that were not regulated in the same way by food restriction,” says Dr. Friedman. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK “So, leptin is doing a lot more than just leading to food intake restriction.” The discovery of these leptin-regu- lated genes offers a glimpse of the complex metabolic machinery con- trolled by leptin. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK The new findings also open a promising pathway for understanding the complexity of lep- tin’s effects on different body tissues, said Friedman. Although researchers know that leptin is produced by fat cells and suppresses appetite by affecting the hypothalamus, the hor- mone-like protein may also trigger metabolic changes in fat and other tissues. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Learning how changes in gene regulation lead to these effects is a goal of future studies in Friedman’s laboratory. Break Through Weight-loss Plateaus with Leptin The lowered levels of leptin associated with initial weight-loss may be offset by supplemental leptin. “No matter how hard you try, it’s often the case that losing the last 10 or 15 pounds of stubborn fat can take forever,” notes health writer Christian Finn. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK This is where supplemental leptin seems to help. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK In an experimental study, after two weeks of leptin treat- ment, leptin-deficient mice unable to produce leptin naturally lost 40 per- cent of their body weight. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK The reduced weight came exclusively from fat, mir- roring results from the human clinical trial. The body fat content of the nor- mal mice receiving leptin, however, also was reduced up to 12 percent. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Food Consumption Reduced and Metabolic Rate Increased Leptin treatment not only reduced food consumption in the mice, but also increased their energy expenditure. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK Four days after starting leptin treat- ment, the mice with the deficient lep- tin production gene consumed about 60 percent less food compared to untreated mice. When the researchers put a group of untreated mice on a low calorie diet in which they were fed only as much food as the treated mice, the food- restricted mice lost less weight. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK This suggests that leptin also increases energy expenditure. KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK In short, these early studies suggest that leptin is released from fat stores in response to a rise in fat stores—trig- gering appetite suppression and an increase in energy expenditure. Thus, says one weight-loss expert, “Think of leptin a little like a thermostat—reduc- ing food intake and increasing the metabolic rate when your body fat moves beyond a set point.” y KcOiUvzgOnclWYkuKNRegIGfsYTIIIVgCHDgEAzdKfwIXK


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